New antiserum against Ki-67 antigen suitable for double immunostaining of paraffin wax sections.
نویسندگان
چکیده
منابع مشابه
Prognostic significance of Ki-67 antigen immunostaining (MIB-1 monoclonal antibody) in ovarian cancer.
OBJECTIVE To assess the potential usefulness of Ki-67 antigen expression as a predictor of outcome in ovarian cancer through the analysis of MIB-1 monoclonal antibody reactivity. METHODS Cell proliferation and clinicopathologic variables were assessed in 26 patients with primary epithelial ovarian cancer who had undergone exploratory laparotomy. The expression of primary tumor proliferation r...
متن کاملUtility of Ki-67 (MIB-1) Immunostaining in Cervical Biopsies
Nearly every pathologist that practices in a community hospital setting struggles with the accurate and reproducible identification of HPV effect and dysplasia in the female genital tract, and recognizes the fact that in certain situations these conditions can be very difficult to distinguish from reactive changes. Last month in the ProPath "Focus on Immunohistochemistry", we reviewed the poten...
متن کاملUltrarapid Ki-67 immunostaining in frozen section interpretation of gliomas.
BACKGROUND Astrocytic tumours, the most common gliomas, are often classified intraoperatively using standard morphological staining. The final diagnosis and grading of gliomas on paraffin wax sections is often assisted by Ki-67 immunohistochemistry, but standard immunostaining protocols take too long to be used intraoperatively. AIMS To investigate a new rapid Ki-67 immunohistochemical test f...
متن کاملImproved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence.
AIMS To develop and immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. METHODS This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and in...
متن کاملP16/Ki-67 Immunostaining is Useful in Stratification of Atypical Metaplastic Epithelium of the Cervix
Cervical metaplastic squamous epithelium exhibiting atypia insufficient for a diagnosis of cervical intraepithelial neoplasia (CIN) is usually reported as "atypical squamous metaplasia" (ASM). Stratification impacts treatment since the differential is often between reactive and high grade CIN (CIN II, III). Diagnosis with H&E is associated with low intra/interobserver concurrence. P16/Ki-67 imm...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1993
ISSN: 0021-9746
DOI: 10.1136/jcp.46.12.1080